Effect of Semen Leaching and Soybean Lecithin Levels in Tris Extenders on the Quality of Preserved Sheep Spermatozoa at 5oC Temperature
), Marsudi Marsudi(2), Deka Uli Fahrodi(3), Hendro Sukoco(4),
(1) Universitas Tadulako
(2) Universitas Sulawesi Barat
(3) Universitas Sulawesi Barat
(4) Universitas Sulawesi Barat
Corresponding Author
Abstract
This study aims to study the leaching of semen and the level of soy bean lecithin (Soybean lecithin) in relation to the quality of sheep spermatozoa preserved at 5OC. The study used a Complete Randomized Design (RAL) factorial pattern of 2 x 5 x 3. As the first factor is cement washing (P), consisting of P0 = Fresh cement without washing and P1 = Fresh cement undergoes a washing process. The second factor is the level of soy bean lecithin (L), consisting of L0, L1, L2, L3, and L4. Independent variables are cement washing (P) and soybean lasitin levels (L). As a dependent variable is the quality of spermatozoa at a storage temperature of 5⁰C, which consists of progressive motility, viability, abnormality and intergrity of the spermatozoa membrane. The results of the study obtained that the treatment of cement leaching and soy bean lecithin levels together did not show any noticeable interaction, however, single-seeded lecithin levels showed markedly different influences (P≤0.05) and the washing treatment showed no noticeably different influence on all quality parameters of sheep spermatozoa during five days of storage at 5⁰C. The use of soy bean lecithin as a component of sheep cement extenders is effective against samples of unwashed cement or washed cement. The best quality of sheep spermatozoa was obtained at the treatment of 3% soy bean lecithin levels in Tris extenders against washed and unwashed semen preserved for five days of storage at 5oC with an average progressive motility percentage of 63.18%; viability 72.20%; abnormality 12.43%; and membrane integrity of 72.92%.
Keywords
References
Ashmawy, T.A.M., A.A. Sallam, A.E. Abd El-Khalek, B.E.El-Saidy and M.G. Gabr. 2010. Recovery and fertilization rates of goat spermatozoa as affected by different levels of egg yolk, dilution rates, freezing method and months of the year. Egypt J. Sh. G. Sci. 5: 283 – 293.
Ax, R.L., M.Dally, B.A.Didion, R.W.Lenz, C.C.Love, D.D.Varner, B.Hafez, and M.E.Bellin. 2000. Semen evaluation. In: Hafez, B and E.S.E.Hafez (eds), Reproduction in Farm Animals. 7th ed. pp: 365-375. Lea and Febiger, Philadelphia.
Ayu D.K. 2012. Pengaruh Berbagai Bahan Pengarcer Terhadap Motilitas, Viabilitas dan Membran Plasma Utuh Sperma Sapi Friesian Holstein Post-Thawing (Tesis). Program Magister Fakultas Kedokteran Hewan. Universitas Airlangga- Surabaya.
Bearden, H.J., J.W.Fuquay, and S.T. Willard. 2004. Applied Animal Reproduction (6th ed). Prentice-Hall, Inc. United States of America. 133 – 203.
Bousseau, S., J.P. Brillard, B.M. Le Guine, and M. Lechat. 1998. Comparison of bacteriological quantics of various egg yolk sources and the in-vitro and in-vivo fertilizing potential of bovine sperm frozen in egg yolk or lecithin-based dilution. Theriogenology. 50: 699-706.
Chemineau, P., Y.Cagnie, P.Orgeaur and J.C.Vallet. 1991. Training Manual on Artificial Insemination in Sheep and Goat. Food and Agricultural Organization of the United Nation, Roma. 27 – 161.
Counsel, M., R.Bellinge and P.Burton. 2004. Vitality of oligozoospermic semen samples is improved by both swim up and density gradient centrifugation before cryopreservation. J. Assis. Reprod. & Gen. 21: 137-142.
Erickson, M.C. 2002. Chemistry and function of phospholipids. In: Akoh, C.C and D.B.Min, eds. Food Lipids. Chemistry, Nutrition, and Biotechnology. 2nd Ed. pp:41-62. Marcel Dekker, Inc. New York.
Evans, G and W.M.C.Maxwell. 1987. Salamon’s Artificial Insemination of Sheep and Goats. Butherworths Pty Limited, Sidney, Boston, London, Durban, Singapore and Wellington. 22 – 164.
Garner, D.L. and E.S.E.Hafez. 2000. Spermatozoa and seminal plasma. In: Hafez, B and E.S.E.Hafez, eds. Reproduction in Farm Animals. 7th Ed. pp: 96-111. Lea and Febiger, Philadelphia.
Immelda, K.H., Suherni, S., Ira, S.Y., 2019. Pengaruh Bahan Pengencer Sari Kacang Keddelai (Glycine Max) Terhadap Viabilitas dan Nekrosis Spermatozoa Domba Sapudi.
Jayendran, R.S. and L.J.D. Zaneveld. 1986. Instruction for hypoosmotic swelling (HOS) test. In: Zaneveld, L.J.D. and D.L. Fulgham, eds. pp. 1 -10. Short course: Male Reproduction/Andrology and Non Hormonal Contraseption
Lee, H.L., S.H.Kim, D.B.Ji and Y.J.Kim. 2009. A comparative study of sephadix, glass woll and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine sperm. J. Vet. Sci. 10: 249-255.
Marcus. 2010. Steps involved in artificial insemination. http://www.ivf-infertility/ insemination/intrauterine/insemination9.php.
Mc Clure, R.D., L. Nunes and R. Tom.1989. Sperm manipulation: Improved sperm recovery and function with two layer percoll gradient. Fertility and Sterility. 51: 5 - 11.
Partodihardjo, S. 1992. Ilmu Reproduksi Hewan. Penerbit Mutiara, Jakarta. 519 – 543.
Perez-Pe, R., T.Muino-Blanco and J.A.Cebrian-Perez. 2001. Sperm washing method alters the ability of seminal plasma proteins to revert the cold-shock damage on ram sperm membrane. International J. Androl. 24: 352-359.
Rizal, M. dan Herdis. 2008. Inseminasi Buatan pada Domba. Penerbit: PT. Rineka Cipta. Jakarta. 10 – 56.
Rizal, M., Herdis, M. Surachman, dan W.M. Mesang-Nelley. 2008. Pengaruh plasma semen domba priangan terhadap daya hidup spermatozoa kambing peranakan etawah yang disimpan pada suhu 3–5⁰C. J. Ilmu Ternak dan Veteriner Departemen Pertanian 13: 23-29
Roldan, E.R.S. and C. Fragio. 1993. Phospholipase A2 activation and subsequent exocytosis in the Ca2+/Ionophore-induced acrosome reaction of ram spermatozoa. J. Biol. Chem. 268: 13962-13970.
Salmin. 2000. Pengaruh Kadar Gliserol dalam Pengencer Susu Skim dan Lama Ekuilibrasi Terhadap Kualitas Semen Domba Pasca Pembekuan. Tesis. Program Pascasarjana Universitas Padjadjaran, Bandung.
Salmin. 2002. Lama ekuilibrasi pengencer bergliserol pada proses pembekuan semen domba. J. Agroland. 9: 284-288.
Sasmita, E. 2017. Pengaruh Pengencer Sari Kacang Kedelai dengan Konsentrasi yang Berbeda Terhadap Kualitas Semen Sapi Bal. Skripsi thesis, Universitas Islam Negeri Sultan Syarif Kasim Riau
Scott, T.W. and R.M.C. Dawson. 1968. Metabolism of phospholipids by spermatozoa and seminal plasma. Biochem. J. 108: 457 – 463.
Shurtleff, W. and A.Aoyagi. 2007. History of Soy Lecithin. Soyinfo Center, Lafayette, California. p: 1-2.
Somfai,T., S.Bodo, S.Nagy, A.B.Papp, J.Ivancsics, B.Baranyai, E.Gocza and A.Kovacs. 2002. Effect of swim up and Percoll treatment on viability and acrosome integrity of frozen-thawed bull spermatozoa. Reprod. Dom. Anim. 37: 285-290.
Toelihere, M.R. 1985b. Inseminasi Buatan pada Ternak. Penerbit Angkasa, Bandung. 46–164
Article Metrics
Abstract View
: 136 times
Download : 137 times
DOI: 10.57235/qistina.v1i2.172
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Salmin Salmin, Marsudi Marsudi, Deka Uli Fahrodi, Hendro Sukoco
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
